Health & Environmental Research Online (HERO)


Print Feedback Export to File
4265300 
Journal Article 
Assimilation of nitrogen from nitrite and trinitrotoluene in Pseudomonas putida JLR11 
Caballero, A; Esteve-Núñez, A; Zylstra, GJ; Ramos, JL 
2005 
Yes 
Journal of Bacteriology
ISSN: 0021-9193
EISSN: 1098-5530 
AMER SOC MICROBIOLOGY 
WASHINGTON 
187 
396-399 
English 
Pseudomonas putida JLR11 releases nitrogen from the 2,4,6-trinitrotoluene (TNT) ring as nitrite or ammonium. These processes can occur simultaneously, as shown by the observation that a nasB mutant impaired in the reduction of nitrite to ammonium grew at a slower rate than the parental strain. Nitrogen from TNT is assimilated via the glutamine syntethase-glutamate synthase (GS-GOGAT) pathway, as evidenced by the inability of GOGAT mutants to use TNT. This pathway is also used to assimilate ammonium from reduced nitrate and nitrite. Three mutants that had insertions in ntrC, nasT, and cnmA, which encode regulatory proteins, failed to grow on nitrite but grew on TNT, although slower than the wild type. 
IRIS
• Nitrate/Nitrite
     Supplemental LitSearch Update 1600-2015
          PubMed
          WoS
          New to project