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Citation
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HERO ID
4265300
Reference Type
Journal Article
Title
Assimilation of nitrogen from nitrite and trinitrotoluene in Pseudomonas putida JLR11
Author(s)
Caballero, A; Esteve-Núñez, A; Zylstra, GJ; Ramos, JL
Year
2005
Is Peer Reviewed?
Yes
Journal
Journal of Bacteriology
ISSN:
0021-9193
EISSN:
1098-5530
Publisher
AMER SOC MICROBIOLOGY
Location
WASHINGTON
Volume
187
Issue
1
Page Numbers
396-399
Language
English
PMID
15601726
DOI
10.1128/JB.187.1.396-399.2005
Web of Science Id
WOS:000226390400044
Abstract
Pseudomonas putida JLR11 releases nitrogen from the 2,4,6-trinitrotoluene (TNT) ring as nitrite or ammonium. These processes can occur simultaneously, as shown by the observation that a nasB mutant impaired in the reduction of nitrite to ammonium grew at a slower rate than the parental strain. Nitrogen from TNT is assimilated via the glutamine syntethase-glutamate synthase (GS-GOGAT) pathway, as evidenced by the inability of GOGAT mutants to use TNT. This pathway is also used to assimilate ammonium from reduced nitrate and nitrite. Three mutants that had insertions in ntrC, nasT, and cnmA, which encode regulatory proteins, failed to grow on nitrite but grew on TNT, although slower than the wild type.
Tags
IRIS
•
Nitrate/Nitrite
Supplemental LitSearch Update 1600-2015
PubMed
WoS
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