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Citation
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HERO ID
4266727
Reference Type
Journal Article
Title
The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds
Author(s)
Chu, HH; Car, S; Socha, AL; Hindt, MN; Punshon, T; Guerinot, ML
Year
2017
Is Peer Reviewed?
1
Journal
Scientific Reports
EISSN:
2045-2322
Volume
7
Issue
1
Page Numbers
11024
Language
English
PMID
28887568
DOI
10.1038/s41598-017-11250-9
Web of Science Id
WOS:000409841100086
Abstract
Understanding how seeds obtain and store nutrients is key to developing crops with higher agronomic and nutritional value. We have uncovered unique patterns of micronutrient localization in seeds using synchrotron X-ray fluorescence (SXRF). Although all four members of the Arabidopsis thaliana Mn-CDF family can transport Mn, here we show that only mtp8-2 has an altered Mn distribution pattern in seeds. In an mtp8-2 mutant, Mn no longer accumulates in hypocotyl cortex cells and sub-epidermal cells of the embryonic cotyledons, but rather accumulates with Fe in the cells surrounding the vasculature, a pattern previously shown to be determined by the vacuolar transporter VIT1. We also show that MTP8, unlike the other three Mn-CDF family members, can transport Fe and is responsible for localization of Fe to the same cells that store Mn. When both the VIT1 and MTP8 transporters are non-functional, there is no accumulation of Fe or Mn in specific cell types; rather these elements are distributed amongst all cell types in the seed. Disruption of the putative Fe binding sites in MTP8 resulted in loss of ability to transport Fe but did not affect the ability to transport Mn.
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