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4267779 
Journal Article 
Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples 
Pîrlea, S; Puiu, M; Răducan, A; Oancea, D 
2017 
Yes 
International Journal of Legal Medicine
ISSN: 0937-9827
EISSN: 1437-1596 
131 
2 
323-331 
English 
27595149 
In this study, it was demonstrated that the DNA Chelex extraction combined with the permanganate assisted-oxidation is highly efficient in removing the PCR inhibitors often found in clothing materials, such as phthalocyanine. The extraction assays were conducted in saliva, blood and epithelial cells samples mixed with three oxidation-resistant dye copper(II) α-phthalocyanine, copper(II) β-phthalocyanine and tetrasulfonated copper(II) β-phthalocyanine. After DNA amplification, all samples were able to provide full DNA profiles. The permanganate/Chelex system was tested further on denim-stained samples and displayed the same ability to remove the PCR inhibitors from the commercial textile materials.