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4270167 
Journal Article 
Mn3+-saturated bovine lactoferrin as a new complex with potential prebiotic activities for dysbiosis treatment and prevention - On the synthesis, chemical characterization and origin of biological activity 
Spiewak, K; Majka, G; Pilarczyk-Zurek, M; Nowak, PM; Wozniakiewicz, M; Pietrzyk, P; Korzeniak, T; Stochel-Gaudyn, A; Fyderek, K; Strus, M; Brindell, M 
2017 
Yes 
Journal of Functional Foods
ISSN: 1756-4646 
38 
264-272 
WOS:000414890500026 
A new bioactive compound, Mn3+-saturated bovine lactoferrin (Mn-2'-bLf) was obtained. It was stable under physiological conditions (pH approximate to 7.5) although gradually releases Mn2+ ions under the simulated intestinal conditions (pH approximate to 6.5). This feature might be utilized for controlled delivery of Mn2+ ions to biological targets, among others to the probiotic strains of lactic acid bacteria whose growth is manganese dependent. The Mn3+ modified bLf stimulates the growth of standard Lactobacillus plantarum and Lactobacillus rhamnosus, as well as the strains isolated from commercially available probiotic preparations, while the naturally occurring Fe-free and Fe-saturated forms of this protein are inactive. The uptake of manganese by Lactobacilli from Mn-2-bLf added to culture supernatant was confirmed. Mn-2-bLf was non-cytotoxic for Caco-2, CT-26 and HT-29 intestinal epithelial cells even up to 10 mg/ml concentration. Mn-2-bLf due to stimulation of the growth of Lactobacilli and lack of toxicity, can be considered as an innovative ingredient of the prebiotic composition. (C) 2017 Elsevier Ltd. All rights reserved. 
Lactobacillus; Dysbiosis; Manganese; Lactoferrin; Prebiotic; Probiotic