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4305093 
Journal Article 
Evaluation of bioactive potential of an Aloe vera sterol extract 
Bawankar, R; Deepti, VC; Singh, P; Subashkumar, R; Vivekanandhan, G; Babu, S 
2013 
1 
Phytotherapy Research
ISSN: 0951-418X
EISSN: 1099-1573 
27 
6 
864-868 
English 
22899575 
WOS:000320114500011 
We prepared a crude gel material from Aloe vera succulent leaf tissues. The ethanolic extract of lyophilized A. vera gel was used for the GC-MS analysis. Hexadecanoic acid (22.22%) was identified as major compound. Sitosterol and stigmasterol were found to be 2.89% and 2.1% in the extract. HPLC analysis was carried out to confirm the presence of stigmasterol. The concentration of sterol extract needed to scavenge DPPH free radical by 50% was calculated as 5.2 mg mL(-1). In the FRAP assay, the sterol extract showed significant hydroxyl radical scavenging in a dose-dependent manner (IC50 value 1.17 µg mL(-1)). Concentration of the sample required to reduce lipid peroxidation was found to be 4.18 µg mL(-1), and the extract also possessed acetylcholinesterase activity (IC50 - 5.26 µg mL(-1)). Catalase activity was 0.196 μM H2 O2 decomposed min(-1) µg(-1) protein, whereas the peroxidase activity was 17.01 μM of pyragallol oxidized min(-1) µg(-1) protein. The extract recorded higher activity against growth of S. greseus and C. albicans in the experiments carried out to determine antibacterial and antifungal activity, respectively.