Health & Environmental Research Online (HERO)


Print Feedback Export to File
4373487 
Journal Article 
Rapid naked eye detection of alkaline phosphatase using alpha-MoO3-x nano-flakes 
Pandey, S; Sharma, AK; Sharma, KH; Nerthigan, Y; Khan, MS; Hang, DaRen; Wu, HuiFen 
2018 
1 
Sensors and Actuators B: Chemical
ISSN: 0925-4005
EISSN: 1873-3077 
254 
514-518 
WOS:000413308000063 
We present a novel platform enabled for rapid naked eye detection of alkaline phosphatase using two-dimensional alpha-MoO3-x nano-flakes as reporter molecule as first of its kind report. The detection system involved alpha-MoO3-x nano-flakes and zinc oxide as the primary constituents followed by the introduction of alkaline phosphatase. The rapid transformation of alpha-MoO3-x (blue) to alpha-MoO3 (colorless) in presence of alkaline phosphatase with zinc as a cofactor is an indication for the presence of the enzyme. The sensitivity of the current biosensor was found to reach 0.1 mu M of enzyme concentration. The stability and selectivity of the novel biosensor has been tested at different pH and with a wide range of analytes in the serum. (C) 2017 Elsevier B.V. All rights reserved. 
alpha-MoO3-x nano-flakes; Alkaline phosphatase; Hydrogen peroxide; Naked eye detectiona 
IRIS
• Molybdenum
     Litsearch 2018
          WOS