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HERO ID
4453028
Reference Type
Journal Article
Title
Chiral interactions of the drug propranolol and α1-acid-glycoprotein at a micro liquid-liquid interface
Author(s)
Lopes, P; Kataky, R
Year
2012
Is Peer Reviewed?
Yes
Journal
Analytical Chemistry
ISSN:
0003-2700
EISSN:
1520-6882
Publisher
American Chemical Society
Book Title
Anal Chem.
Volume
84
Issue
5
Page Numbers
2299-2304
Language
English
PMID
22250754
DOI
10.1021/ac2029425
Web of Science Id
WOS:000301021400030
URL
https://search.proquest.com/docview/2000282717?accountid=171501
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Abstract
The investigation of chiral interactions of drugs with plasma proteins is of fundamental importance for drug efficacy and toxicity studies. In this paper, we demonstrate a simple liquid-liquid interface procedure for investigating chiral interactions. Chiral discrimination of the enantiomers of a basic drug, propranolol, was achieved at a micro liquid-liquid interface, using α(1)-acid-glycoprotein (AGP) as a chiral acute phase plasma protein. When the protein is added to an aqueous phase containing the enantiomers of propranalol hydrochloride, the binding of (S)- and (R)-propranolol hydrochloride to the protein results in a decrease in the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) current responses corresponding to the decrease in transfer of propranolol at an aqueous-1,2-dichloroethane interface. This decrease is a consequence of the complexation of the drug and the protein. The complex drug-protein does not transfer across the interface nor changes the transfer potential of the uncomplexed form of propranolol enantiomers. The bound concentration of propranolol enantiomers in the presence of AGP was found to be greater for (S)-propranolol than (R)-propranolol for solutions containing constant concentrations of AGP (50 μM). Scatchard analysis yielded association constants of 2.7 and 1.3 × 10(5) M(-1) for (S)- and (R)-propranolol, respectively.
Keywords
article; blood proteins; drug interactions; enantiomers; propranolol; toxicity testing
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