Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
4454139
Reference Type
Journal Article
Title
Comparison of different inactivation methods on the stability of Indian vaccine strains of foot and mouth disease virus
Author(s)
Sarkar, A; Tamil Selvan, RP; Kishore, S; Ganesh, K; Bhanuprakash, V
Year
2017
Volume
48
Page Numbers
10-23
Language
English
PMID
28645721
DOI
10.1016/j.biologicals.2017.06.004
Web of Science Id
WOS:000407535800003
Abstract
In this study, the efficiency of binary ethyleneimine (BEI) in combination with formaldehyde (FA) and glutaraldehyde (GTA) in inactivating the Indian FMDV vaccine strains is compared. The acceptable safety of virus inactivation was faster and the inactivation rates were increased many-folds with combination of inactivants than BEI alone. FMDV A was inactivated rapidly than the other two serotypes with BEI + FA combination. Inactivation plots were linear for all the serotypes irrespective of inactivation process. Further, the integrity studies on 146S using serotype specific ELISA indicated no significant change in the antigenic mass of all the serotypes throughout the inactivation process. However, the loss of 146S antigen occurred in the subsequent steps of downstream processing. Further, the studies on intactness of viral RNA using real time PCR indicated the amplification of 1D gene sequences in all the preparations of timed samples irrespective of serotypes/inactivation process. Further, inactivated virus preparation (146S) was more stable at lower temperatures for all the serotypes/inactivation process. Among the combinations of inactivants, BEI + FA out performed compared to BEI + GTA and BEI in terms of inactivation rates, 146S yield and its storage stability, irrespective of the serotypes.
Tags
IRIS
•
Formaldehyde
HAWC
Nervous system
Excluded
Search Update 2016-2017
Nervous system effects
WoS
Search Update 2018-2021
2017-2018 LitSearch
Nervous System Effects
Search Update
WOS
Pulmonary Function
Search Update
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity