US EPA

4455105 

Journal Article 

Massively parallel DNA sequencing from routinely processed cytological smears 

Piqueret-Stephan, L; Marcaillou, C; Reyes, C; Honoré, A; Letexier, M; Gentien, D; Droin, N; Lacroix, L; Scoazec, JY; Vielh, P 

2016 

WILEY 

HOBOKEN 

124 

4 

241-253 

English 

26505273 

10.1002/cncy.21639 

WOS:000374338400005 

http://doi.wiley.com/10.1002/cncy.21639
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BACKGROUND: Data generated by next-generation sequencing technologies have a pivotal role in precision medicine. These high-throughput techniques are preferentially performed on fresh tissue, but there is an increasing need for protocols adapted to materials derived from formalin-fixed, paraffin-embedded tissue and cytology specimens.

METHODS: The aim of this work was to show that cytological material collected from archival smears processed for routine diagnoses could be used for massively parallel sequencing and array-based genomic analysis for further studies.

RESULTS: As a proof of concept, data obtained from May-Grünwald Giemsa- and Diff-Quik-stained archival smears were shown to be in keeping with those obtained from matched frozen controls.

CONCLUSIONS: The quality of DNA extracted from routinely processed smears is compatible with the multitargeted sequencing of a large series of genes of interest with methods such as array-based genomic analysis and whole-exome sequencing.