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4469170 
Journal Article 
Glutamate-induced cytotoxicity in PC12 pheochromocytoma cells: role of oxidation of phospholipids, glutathione and protein sulfhydryls revealed by bcl-2 transfection 
Tyurin, VA; Tyurina, YY; Quinn, PJ; Schor, NF; Balachandran, R; Day, BW; Kagan, VE 
1998 
1 
Molecular Brain Research
ISSN: 0169-328X 
ELSEVIER SCIENCE BV 
Netherlands 
60 
2 (October 1 
270-281 
English 
9757062 
WOS:000076433600013 
Incubation of mock-transfected PC12 rat pheochromocytoma cells (PC12) for 2 h with increasing concentrations of glutamate caused progressive loss of viability (e.g., 67% with 15 mM glutamate). In contrast, the viability of bcl-2-transfected cells (PC12/bcl-2) was unaffected by glutamate. Neither PC12 nor PC12/bcl-2 cells showed a significant incidence of apoptosis in response to glutamate. Conventional phospholipid analysis by high-performance TLC and phosphorous determination showed no significant changes in the phospholipid composition of either cell line incubated with 5 mM glutamate. The peroxyl radical initiator 2,2'-azobis(2,4-dimethylvaleronitrile) caused a pronounced loss of all major phospholipid classes in PC12 cells, but no loss of cell viability. No phospholipid peroxidation was detected in PC12/bcl-2 cells incubated with
Index Medicus; 1998)