Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
4469422
Reference Type
Journal Article
Title
Histology of the Ovary of the Laying Hen (Gallus domesticus)
Author(s)
Apperson, KD; Bird, KE; Cherian, G; Löhr, CV
Year
2017
Volume
4
Issue
4
Language
English
PMID
29232906
DOI
10.3390/vetsci4040066
Abstract
The laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and proximal oviduct to establish baselines from which lesions associated with ovarian cancer can be more readily identified. Ovaries and oviducts were collected from 18-month-old laying hens (n = 18) and fixed in 10% neutral buffered formalin. Hematoxylin- and eosin-stained sections were examined by light microscopy. Both post-ovulatory follicular regression and atresia of small follicles produce remnant clusters of vacuolated cells with no histological evidence that scar tissue persists. Infiltrates of heterophils are associated with atresia of small follicles, a relationship not previously documented in laying hen ovaries. Because these tissues can be mistaken for cancerous lesions, we present a detailed histological description of remnant Wolffian tissues in the laying hen ovary. Immunohistochemical staining for pancytokeratin produced a positive response in ovarian surface epithelium and staining for vimentin produced a positive response in granulosa cells of follicles. Epithelial cells lining glands of the remnant epoöphoron had a positive response to both pancytokeratin and vimentin, a result also observed in women.
Tags
IRIS
•
Formaldehyde
HAWC
Reproduction and development
Excluded
Search Update 2016-2017
Reproductive and Developmental effects
PubMed
Search Update 2018-2021
Animal UR Cancer Studies
PubMed
2017-2018 LitSearch
Animal UR Cancer Studies
Pubmed
Search Update
Reproductive and Developmental Effects
Pubmed
Search Update
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity