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4541385 
Journal Article 
Ascorbic acid as a reducing agent for phosphorus analysis by colorimetry 
Santos, L; da Silva, LS; Griebeler, G 
2014 
Yes 
Ciencia Rural
ISSN: 0103-8478 
UNIV FEDERAL SANTA MARIA 
SANTA MARIA 
44 
6 
1015-1018 
English; Portuguese 
WOS:000336964100010 
In soil laboratory testing, the acid 1-amino-2-naphthol-4-sulfonic (ANS) is used as reducing agent to determine P by UV/Visible spectrometry. However, there are risks to laboratory technician healthy and an alternative is the use of ascorbic acid (AA). But, changes in the analytical protocol may induce variations in results, been necessary to standardize and calibrate. The objective of this study was to evaluate the ANS and AA in extracts of Mehlich-1 and 3 in ranges 15, 30, 45, 60 and 120min of reaction. There was no difference in P concentration determined by both reducers when compared within each extractor. Ascorbic acid can substitute the ANS solution for P determination and the suggested reaction interval for colorimetric quantification is between 30 and 60min for both substances. 
soil test; reducing agents; Mehlich-1; Mehlich-3