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454617 
Journal Article 
Gene expression of circulating tumour cells and its correlation with tumour stage in breast cancer patients 
Boke, E; Orth, K; Gerber, PA; Lammering, G; Mota, R; Peiper, M; Matuschek, C; Budach, W; Rusnak, E; Shaikh, S; Dogan, B; Prisack, HB; Bojar, H 
2009 
Yes 
European Journal of Medical Research
ISSN: 0949-2321 
14 
8 
359-363 
English 
Background: Breast cancer (BC) represents one of the leading causes of cancer related deaths worldwide. New tools for diagnostic staging and therapeutic monitoring are needed to improve individualized therapies and improve clinical outcome. The analyses of circulating turnout cells may provide important prognostic information in the clinical setting. Materials and Methods: Circulating tumour cells (CTC) of 63 BC patients were isolated from peripheral blood (PB) through immunomagnetic separation. Subsequently, RT-PCR or mPCR for the genes ga733.2, muc-1, c-erbB2, mgb-1, spdef and c-erbB2 were performed. Subsequently, expression data were correlated with the tumour stages. Fourteen health), individuals served as controls. Results: Significant correlations with tumour stages were found in single gene analyses of ga733.2, muc-1 and in multi-gene analyses of ga733.2/muc-1/mgb1/ spdef. Furthermore, a significant correlation of Ca 15-3 and all studied genes was also observed. Conclusion: Herein, we demonstrated a positive correlation of a gene signature consisting of ga733.2,muc-1, mgb1 and spdef and advanced stages of BC. Moreover, all studied genes and gene patterns revealed a significant correlation with Ca 15-3 positive cases. 
Mamma carcinoma; gene signature; real-time-PCR; mammary-tumors; blood; carcinoma; identification; induction; ca-15-3; mucin; mice