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4582014 
Journal Article 
On-line Concentration and Separation of Parabens by Micellar Electrokinetic Chromatography Using Polymer Solutions 
Wu, CWei; Lee, JanYee; Hu, ChoC; Chiu, TaiC 
2014 
Yes 
Journal of the Chinese Chemical Society
ISSN: 0009-4536 
61 
4 
453-460 
WOS:000333804700008 
Simultaneous separation and quantification of seven parabens commonly used as preservatives in cosmetic products, by micellar electrokinetic chromatography with a stacking technique has been demonstrated. An effective on-line concentration strategy involving a combination of sweeping and the use of polymer solutions is a key feature of the proposed method, which successfully determined individual parabens. The analysis parameters such as injection time, pH and concentration of phosphate solution, and concentration of sodium dodecyl sulphate (SDS) and poly(ethylene oxide) (PEO) were examined. The optimum conditions were found to be as follows: a 15 mM phosphate solution (pH 9.5) containing 20 mM SDS for filling the capillary, and for the separation electrolytes, 0.100% PEO (8 MDa) added to the phosphate and SDS solution of the same composition as for the capillary. The entire analysis process was completed in 13 min and a 930-2200-fold enhancement factor was achieved. The LODs (S/N = 3) for this approach were in the range from 4.32 to 7.78 nM. The linear range for each paraben was between 50 nM and 5.0 M (R-2 > 0.990). The optimized method was then successfully applied to the determination of parabens in commercial cosmetic products. 
Micellar electrokinetic chromatography; Parabens; Poly(ethylene oxide); Sodium dodecyl sulphate; Stacking