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HERO ID
4582014
Reference Type
Journal Article
Title
On-line Concentration and Separation of Parabens by Micellar Electrokinetic Chromatography Using Polymer Solutions
Author(s)
Wu, CWei; Lee, JanYee; Hu, ChoC; Chiu, TaiC
Year
2014
Is Peer Reviewed?
Yes
Journal
Journal of the Chinese Chemical Society
ISSN:
0009-4536
Volume
61
Issue
4
Page Numbers
453-460
DOI
10.1002/jccs.201300124
Web of Science Id
WOS:000333804700008
Abstract
Simultaneous separation and quantification of seven parabens commonly used as preservatives in cosmetic products, by micellar electrokinetic chromatography with a stacking technique has been demonstrated. An effective on-line concentration strategy involving a combination of sweeping and the use of polymer solutions is a key feature of the proposed method, which successfully determined individual parabens. The analysis parameters such as injection time, pH and concentration of phosphate solution, and concentration of sodium dodecyl sulphate (SDS) and poly(ethylene oxide) (PEO) were examined. The optimum conditions were found to be as follows: a 15 mM phosphate solution (pH 9.5) containing 20 mM SDS for filling the capillary, and for the separation electrolytes, 0.100% PEO (8 MDa) added to the phosphate and SDS solution of the same composition as for the capillary. The entire analysis process was completed in 13 min and a 930-2200-fold enhancement factor was achieved. The LODs (S/N = 3) for this approach were in the range from 4.32 to 7.78 nM. The linear range for each paraben was between 50 nM and 5.0 M (R-2 > 0.990). The optimized method was then successfully applied to the determination of parabens in commercial cosmetic products.
Keywords
Micellar electrokinetic chromatography; Parabens; Poly(ethylene oxide); Sodium dodecyl sulphate; Stacking
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