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4628346 
Journal Article 
Effects of Arsenic on Flavonoids Accumulation and Metabolic Pathway in Main Roots of Panax notoginseng 
Zu, Y; Cheng, S; Li, Z; Mei, X; Wu, J 
2018 
Yes 
International Journal of Agriculture and Biology
EISSN: 1560-8530 
20 
6 
1375-1381 
WOS:000433197100017 
Panax notoginseng (Burk.) F.H. Chen is one of herbaceous, perennial medicinal plants. Flavonoids are the key medicinal compositions of P. notoginseng, but its regulating molecular mechanism under arsenic stress was not elucidated. The contents of flavonoids and arsenic, its relative key enzymes PAL (phenylalanine ammonia lyase) and CHS (chalcone synthase) in roots of P. notoginseng under different arsenic treatment levels (As5+: 0, 20, 140 mg kg(-1)) were determined at early flowering stage. Meanwhile, the transcriptomes of P. notoginseng roots were compared using RNA sequencing (RNA-seq) to identify the molecular mechanism and candidate genes involved in flavonoids. The qRT-PCR assays were implemented to verify the expression patterns of nine representatives of regulated DEGs. The results showed that: (1) Flavonoid contents increased with 20 mg kg-1 arsenic treatment, while decreased with 140 mg kg(-1) arsenic treatment. PAL activities increased and CHS activities decreased with increase in arsenic treatment concentrations. (2)Genes relating to arsenic stress showed distinct patterns with biosynthesis of secondary metabolism. We identified metabolism processing associated with flavonoid. (3) Genes of key enzymes relating to the pathway of flavonoid (relative genes: DcCHS2, LOC100221899) biosynthesis were identified. The results would be important in revealing the genetic data that affects flavonoid biosynthesis and provides novel gene resources for P. notoginseng breeding under arsenic stress. (C) 2018 Friends Science Publishers 
Medicinal plant; Heavy metal stress; Differential expressed genes; RNA-sequencing; Secondary metabolism