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4635452 
Journal Article 
Phenotypic Analysis of Alternaria alternata, the Causal Agent of Tobacco Brown Spot 
Wang, H; Huang, Y; Xia, H; Wang, Jin; Wang, M; Zhang, C; Lu, H 
2015 
1 
Plant Pathology Journal
ISSN: 1812-5387
EISSN: 1812-5425 
Asian Network for Scientific Information, 308-Lasani Town Faisal Abad 38090 Pakistan 
14 
2 (0 
79. 
Tobacco brown spot caused by Alternaria alternata is a devastating disease of tobacco worldwide. Phenotypic characterization of the pathogen was investigated to provide some basic information for biology and pathology by using BIOLOG Phenotype Microarray (PM). Using PM plates 1-10, 950 different growth conditions were tested. Results exhibited that the pathogen was able to metabolize 24.74% of tested carbon sources, 85.26% of nitrogen sources, 97.14% of sulfur sources and 89.83% of phosphorus sources. The pathogen presented 274 different nitrogen pathways. It had wide range adaptabilities in osmolytes with up to 10% sodium chloride, up to 6% potassium chloride, up to 5% sodium sulfate, up to 20% ethylene glycol, up to 6% sodium formate, up to 6% urea, up to 12% sodium lactate, up to 200 mM sodium phosphate, up to 100 mM ammonium sulfate, up to 100 mM sodium nitrate and up to 20 mM sodium nitrite. The pathogen showed active decarboxylase activity, whereas no deaminase activity in the presence of various amino acids. 
Amino acids; Nitrogen sources; Growth conditions; Phosphorus; Sodium lactate; potassium chloride; Urea; Carbon sources; Pathogens; Sodium nitrite; sodium sulfate; Adaptability; sodium phosphate; Ammonium sulfate; sulfur sources; Tobacco; sodium formate; Ethylene glycol; Brown spot; Sodium chloride; Nitrogen; sodium nitrate; Alternaria alternata; 2015)