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4668547 
Journal Article 
Cultivation of Nannochloropsis oceanica biomass rich in eicosapentaenoic acid utilizing wastewater as nutrient resource 
Mitra, M; Shah, F; Bharadwaj, SV; Patidar, SK; Mishra, S 
2016 
Yes 
Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 
218 
1178-1186 
English 
27472494 
WOS:000384710500147 
The eicosapentaenoic acid rich marine eustigmatophyte Nannochloropsis oceanica was grown in wastewaters sampled from four different industries (i.e. pesticides industry, pharmaceutical industry, activated sludge treatment plant of municipality sewage and petroleum (oil) industry). Under the wastewater based growth conditions used in this study, the biomass productivity ranged from 21.78±0.87 to 27.78±0.22mgL(-1)d(-1) in relation to freeze dried biomass, while the lipid productivity varied between 5.59±0.02 and 6.81±0.04mgL(-1)d(-1). Although comparatively higher biomass, lipid and EPA productivity was observed in Conway medium, the %EPA content was similarly observed in pesticides industry and municipal effluents. The results highlight the possibility of selectively using wastewater as a growth medium, demonstrating the elevated eicosapentaenoic acid content and biodiesel properties, that complies with the European standards for biodiesel. 
Biodiesel properties; Eicosapentaenoic acid; Nannochloropsis oceanica; Principal component analysis; Wastewater