Health & Environmental Research Online (HERO)


Print Feedback Export to File
4670153 
Journal Article 
Garlic, cilantro and chlorella's effect on liver histoarchitecture changes in Cd-intoxicated Prussian carp (Carassius gibelio) 
Nicula, M; Dumitrescu, G; Pacala, N; Stef, L; Tulcan, C; Dragomirescu, M; Dronca, D; Gherbon, A; Ciochina, LP 
2016 
1 
Romanian Biotechnology Letters
ISSN: 1224-5984 
21 
6 
12062-12070 
WOS:000392108600011 
Bioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the purpose of this study was to compare histologically the aspect of liver tissue of Prussian carps' specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation. 150 Prussian carps, with weight of 10-12 g, were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed). Fragments of liver were removed at the end of experimental period and analyzed in light microscopy and a specific QuickPHOTO Micro 2.2 software has been used for the histological study. Mentioned epithelium suffered evident histo-morphological alterations under the action of Cd while the active principles from garlic, cilantro and chlorella powder have showed chelating and antioxidant potential, cilantro and garlic being the most effective. 
cadmium (Cd) toxicity; fish; liver; histopathology; garlic; cilantro; chlorella