US EPA

4700751 

Journal Article 

Fed batch strategy for biodegradation of monochloroacetic acid by immobilized xantobacter autotrophicus GJ10 in polyacrylamide gel 

Vasileva, E; Petrov, K; Beschkov, V 

2009 

Yes 

Comptes Rendus de l'Académie Bulgare des Sciences
ISSN: 1310–1331
EISSN: 2367–5535 

62 

10 

1241-1246 

English 

WOS:000272107800008 

The biodegradation of monochloroacetic acid (MCA) is an object of many studies because of two main reasons: it possesses a strong toxic effect on water ecosystems itself and takes place as, an intermediate in the process of degradation of the severe chemical pollutant 1,2-dichloroothane (DCE). The strain Xanthobacter autotrophicus GJ10 is known with its ability to degrade DCE through 2-chloroethanol, MCA and glycolate (GLA) pathway.



In the present work a pure culture of X. autotrophicus GJ10 was immobilized in polyacrylamide gel and was tested for its capability to biodegrade high amounts of MCA as a sole carbon source. Aiming to increase the final volume of the MCA destroyed and to overcome the substrate inhibition, a fed batch process performance was applied. In a stirred tank bioreactor under microaerobic conditions, pH 7.01 30 degrees C and agitation 400 rpm degradation of 150 mM MCA for 330 h was achieved. Our study comprises the first report concerning the immobilization of GJ10 aiming high DCE or MCA utilization. The amount of the converted MCA reported here is the hi-hest reached until now. 

biodegradation; monochloroacetic acid; immobilization; Xanthobacter autotrophicus GJ10