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4721966 
Journal Article 
Immobilization of beta-Galactosidase from Aspergillus oryza on Magnetic Poly(GMA-ST) Beads 
Sun, S; Zhao, X; Xu, X 
2013 
Yes 
Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 
ASIAN JOURNAL OF CHEMISTRY 
SAHIBABAD 
25 
1 
381-384 
English 
WOS:000312620300074 
In this study, novel magnetic beads were prepared from glycidylmethacrylate (GMA) and styrene (ST) via suspension polymerization with cyclohexanol and lauryl alcohol as porogenic agent. The magnetic poly (GMA-ST) beads were characterized with scanning electron microscope (SEM), magnetic scales and X-ray spectroscopy. The magnetic susceptibility of the porous poly (GMA-ST) beads was 1.25 x 10(-4) cm(3)/g. Under the optimum conditions, beta-galactosidase was immobilized on the magnetic poly (GMA-ST) beads described above, the results obtained showed that the activity of the immobilized beta-galactosidase reached 412.09 U/g and the activity recovery was 61.32 %. Finally, the basic properties of the immobilized enzyme were determined separately and satisfactory results were obtained in pH stability, thermal stability, operational stability and Michaelis constants K-m. 
Magnetic beads; Poly(GMA-ST); Immobilized beta-Galactosidase; Suspension polymerization