Health & Environmental Research Online (HERO)


Print Feedback Export to File
4736334 
Journal Article 
Evaluation of safety, antimicrobial activity and probiotic properties of Escherichia coli Nissle 1917 isolated from Idli batter 
Ramachandran, C; Rani, RS; Usha, A 
2016 
1 
Research Journal of Biotechnology
ISSN: 0973-6263 
11 
7 
42-49 
WOS:000379744000006 
The present study was undertaken to characterize probiotic efficiency and evaluate the safety of lactose utilizing Escherichia coli Nissle 1917 isolated from frozen idli batter. The strain inhibited the growth of 13 dysentery causing pathogens and shows commensal relationship with 6 lactic acid bacteria (LAB) strains, 4 commercial yeast and bacterial probiotic strains. PCR amplification with gram-negative 16S rRNA primers confirmed Escherichia coli Nissle 1917.



The strain was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It showed efficient auto-aggregation and co-aggregation ability with 6 LAB and 4 commercial probiotic yeast as well as exhibited good hydrophobicity in xylene and toluene. Isolated E. Nissle 1917 (KT000039) has probiotic characteristics similar to LAB and this may be a solution for the steadily increasing demand in food industry which has to overcome the need of efficient probiotics with a wide antimicrobial spectrum. 
Escherichia coli Nissle; probiotics; idli batter; antimicrobial