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4800131 
Journal Article 
The immunoregulation effect of alpha 1-antitrypsin prolong β-cell survival after transplantation 
Wang, Y; Yan, HJ; Zhou, SY; Wang, YS; Qi, H; Deng, CY; Li, FR 
2014 
1 
PLoS ONE
EISSN: 1932-6203 
9 
4 
e94548 
English 
24722487 
has retraction 5790112 The Immunoregulation Effect of Alpha 1-Antitrypsin Prolong beta-Cell Survival after Transplantation (Retraction Article, vol 9, 2014) (Retraction of Vol 9, art no E94548, 2014)
Islet transplantation has considerable potential as a cure for diabetes. However, the difficulties that arise from inflammation and the immunological rejection of transplants must be addressed for islet transplantation to be successful. Alpha 1-antitrypsin (AAT) inhibits the damage on β cells caused by inflammatory reactions and promotes β-cell survival and proliferation. This protein also induces specific immune tolerance to transplanted β cells. However, whether the expression of AAT in β cells themselves could eliminate or decrease immunological rejection of transplants is not clear. Therefore, we established a β cell line (NIT-hAAT) that stably expresses human AAT. Interestingly, in a cytotoxic T lymphocyte (CTL)-killing assay, we found that hAAT reduced apoptosis and inflammatory cytokine production in NIT-1 cells and regulated the Th1/Th2 cytokine balance in vitro. In vivo transplantation of NIT-hAAT cells into mice with diabetes showed hAAT inhibited immunological rejection for a short period of time and increased the survival of transplanted β cells. This study demonstrated that hAAT generated remarkable immunoprotective and immunoregulation effects in a model of β cell islet transplantation for diabetes model.