The occurrence of trace metals in the bovine eye was examined. Silicon (7440213) (Si), barium (7440393) (Ba), iron (7439896) (Fe), copper (7440508) (Cu), molybdenum (7439987) (Mo), silver (7440224) (Ag), tin (7440315) (Sn), manganese (7439965) (Mn), lead (7439921) (Pb), aluminum (7429905) (Al), nickel (7440020) (Ni), and zinc (7440666) (Zn) were measured spectrophotometrically in various parts of the eyes. Samples were prepared by dissolution in acid or by ashing. Values were expressed as milligram percent of tissue wet weight. Si was highest in the cornea (1.3) and lowest in the sclera (0.2). Ba was highest in the urea (22.0). No Ba was found in the lens, vitreous body, or retina. Fe was highest in the urea (7.1), and lowest in the vitreous body (0.2). Cu was highest in the vitreous body (0.1), and lowest in the urea and retina (0.08). Mo varied from 0.002 to 0.02 in the cornea, 0.001 to 0.06 in the sclera, 0.03 in the lens, 0.0007 to 0.01 in the vitreous body, 0.0015 to 0.5 in the urea, and 0.0075 to 0.08 in the lens. Ag was highest in the retina (0.08), lowest in the cornea (0.03), and varied from 0.006 to 0.06 in the lens. Sn was highest in the lens (0.2). No Sn was found in the sclera, urea, or retina. Mn ranged from 0.005 to 0.1 in the cornea, 0.012 to 0.13 in the sclera, 0.004 to 0.06 in the lens, 0.04 to 0.06 in the vitreous body, 0.02 to 0.1 in the urea, and 0.007 to 0.06 in the retina. Pb was highest in cornea (0.08) and lowest in the sclera and retina (0.02). Al was 0.5 in the cornea, 0.004 to 0.7 in the sclera, 0.5 to 5.6 in the lens, 0.3 to 2.5 in the vitreous body, 0.6 in the urea, and 0.02 to 0.6 in the retina. Ni was highest in the urea (0.15) and lowest in the sclera (0.004). The lens had the highest and lowest Zn values (0.003 to 0.75). No Zn was found in the vitreous body. The percent of water in eye parts ranged from 67.3 in the lens to 98.7 in the vitreous body. The percent of ash ranged from 0.41 in the sclera to 0.82 in the vitreous body and urea. The author concludes that these figures represent baselines for studying the effects of trace metal deficiencies on the eye.