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Citation
Tags
HERO ID
484471
Reference Type
Journal Article
Title
Cell cycle-dependent expression of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis
Author(s)
Joachimiak, E; Pucciarelli, S; Barchetta, S; Ballarini, P; Kaczanowska, J; Miceli, C
Year
2007
Is Peer Reviewed?
1
Journal
Protist
ISSN:
1434-4610
Publisher
ELSEVIER GMBH
Location
MUNICH
Volume
158
Issue
1
Page Numbers
39-50
Language
English
PMID
17023214
DOI
10.1016/j.protis.2006.08.001
Web of Science Id
WOS:000244184400005
Abstract
In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). gamma-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of gamma-tubulin on different MTOCs and during the cell cycle. In Tetrahymena thermophila, the alpha- and beta-tubulin expression is regulated mainly at the transcriptional level, and changes in the ratio of polymerized/unpolymerized tubulin dimers lead to an increase or decrease of alpha- and beta-tubulin transcription. This study deals with the characterization of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Sequence analysis revealed some specific substitutions in nucleotidebinding loops characteristic of the Tetrahymena genus and putative conserved phosphorylation sites located on the external surface of the gamma-tubulin molecule. gamma-Tubulin expression during the cell cycle, in the presence of microtubular poisons and after deciliation, was also characterized. We found that gamma-tubulin mRNA levels are correlated with basal body proliferation and gamma-tubulin nuclear localization. We also found that gamma-tubulin expression changes during anti-microtubular drugs treatment, but does not changes during reciliation. These findings suggest a relationship between the level of unpolymerized tubulin dimers and gamma-tubulin transcription. (c) 2006 Elsevier GmbH. All rights reserved.
Keywords
cell cycle; cytoskeleton; gene expression regulation; tubulin; microtubules; microtubule-organizing centers; beta-tubulin; messenger-rna; basal; bodies; posttranslational modifications; heat-shock; nucleation; alpha; localization; thermophila
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