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4931759 
Journal Article 
The electron-capture gas chromatography of paradichlorobenzene metabolites as a measure of exposure 
Mckinney, JD; Fishbein, L; Fletcher, CE; Barthel, WF 
1970 
Yes 
Bulletin of Environmental Contamination and Toxicology
ISSN: 0007-4861
EISSN: 1432-0800 
5 
4 
354-361 
English 
WOS:A1970H467600012 
Paradichlorobenzene (106467) (PDB) and its metabolites were measured in rabbits by electron capture gas chromatography. New-Zealand-white-rabbits were given a single dose of 0.5 grams per kilogram PDB by stomach tube. Blood samples were taken on days 1, 2, and 3, and urine samples were collected from both test and control rabbits. Blood samples were immediately centrifuged and refrigerated. Urine samples were acidified using hydrochloric-acid. Standard solutions of PDB, 2,5-dichlorophenol (583788) (DCP), and 2,5-dichloroquinol (824691) (DCQ) were used. Gas liquid chromatography (GLC) analysis was conducted using nitrogen as the carrier gas. The peak height of sensitivity for this method was 0.38 nanograms per centimeter (ng/cm) for PDB, 0.55ng/cm for DCP, and 0.06ng/cm for DCQ. Percentage recoveries from spiked urine samples were 78 for PDB, 92 for DCP, and 85 for DCQ. Percentage recoveries from spiked serum samples were 94 for PDB, 87 for DCP, and 0 for DCQ. The authors suggest that this method allows determination of tolerable levels of exposure as measured by excreted metabolites. This method may help to reveal the mode and mechanism of action of PDB and other halobenzenes, and may be broadly applicable to direct GLC determination of chlorinated phenols. 
pesticides