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502387 
Journal Article 
Phylogenetic analysis of P5P-type ATPases, a eukaryotic lineage of secretory pathway pumps 
Moller, AB; Asp, T; Holm, PB; Palmgren, MG 
2008 
1 
Molecular Phylogenetics and Evolution
ISSN: 1055-7903 
46 
2 
619-634 
English 
Eukaryotes encompass a remarkable variety of organisms and unresolved lineages. Different phylogenetic analyses have lead to conflicting conclusions as to the origin and associations between lineages and species. In this work, we investigated evolutionary relationship of a family of cation pumps exclusive for the secretory pathway of eukaryotes by combining the identification of lineage-specific genes with phylogenetic evolution of common genes. Sequences Of P-5 ATPases, which are regarded to be cation pumps in the endoplasmic reticulum (ER), were identified in all eukaryotic lineages but not in any prokaryotic genome. Based on a protein alignment we could group the P-5 ATPases into two subfamilies, P-5A and P-5B that, based on the number of negative charges in conserved trans-membrane segment 4, are likely to have different ion specificities. P-5A ATPases are present in all eukaryotic genomes sequenced so far, while P-5B ATPases appear to be lost in three eukaryotic lineages; excavates, entamoebas and land plants. A lineage-specific gene expansion of up to four different P-5B ATPases is seen in animals. (C) 2007 Elsevier Inc. All rights reserved. 
cation pumps; type V ATPases; P-type ATPase; P-5 ATPase; phylogeny; gene loss; evolution; p-type atpase; unfolded protein response; long-branch attraction; rna-polymerase-ii; genome sequence; giardia-lamblia; saccharomyces-cerevisiae; tetrahymena-thermophila; mitochondrial; remnant; entamoeba-histolytica