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5030412 
Journal Article 
Isopropylbenzene (cumene): A new substrate for the isolation of trichloroethene-degrading bacteria 
Dabrock, B; Riedel, J; Bertram, J; Gottschalk, G 
1992 
Yes 
Archives of Microbiology
ISSN: 0302-8933
EISSN: 1432-072X 
SPRINGER VERLAG 
NEW YORK 
BIOSIS/92/29334 
158 
9-13 
English 
BIOSIS COPYRIGHT: BIOL ABS. Various bacterial isolates from enrichments with isopropylbenzene (cumene), toluene or phenol as carbon and energy sources were tested as to their potential to oxidize trichloroethene (TCE). In contrast to toluene and phenol, all isolates enriched on isopropylbenzene were able to oxidize TCE. Two isolates, strain JR1 and strain BD 1, were identified as Pseudomonas sp. and as Rhodococcus erythropolis, respectively. TCE oxidation was accompanied by the liberation of stoichiometric amounts of chloride. Initial TCE oxidation rate increased proportional to the substrate concentration from 25 to 200 muM TCE. Maximal initial TCE-degradation rates found here were 4 to 5 nmolecreased with time. The two isolates showed a temperature optimum for TCE degradation between 10 and 20?C. In addition to TCE, R. reythropolis BD1 degraded only cis- and trans-dichloroethene whereas Pseudomonas sp. JR1 was able to oxidize also 1,1-dichloroethene, vinyl chloride, trichloroethane, and 1,2-dich 
Benzene Derivatives; Culture Media; Trichloroethylene; 290YE8AR51; 8Q54S3XE7K; Index Medicus; Biodegradation, Environmental; Oxidation-Reduction; Hydrogen-Ion Concentration; Benzene Derivatives -- metabolism; Rhodococcus -- isolation & purification; Pseudomonas -- metabolism; Soil Microbiology; Trichloroethylene -- metabolism; Rhodococcus -- metabolism; Pseudomonas -- growth & development; Rhodococcus -- growth & development; Pseudomonas -- isolation & purification