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5030931 
Journal Article 
In Vivo DNA Adduct Formation by Bisphenol A 
Atkinson, A; Roy, D 
1995 
Yes 
Environmental and Molecular Mutagenesis
ISSN: 0893-6692
EISSN: 1098-2280 
26 
1 
60-66 
WOS:A1995RM10400008 
This study used the phosphorus-32 postlabeling technique to examine the covalent modifications in DNA caused by in-vitro or in-vivo exposure to bisphenol-A (80057) (BPA). Single or multiple doses of 200mg/kg BPA were given to male CD-1-rats to produce two major and several minor adducts in liver DNA. The two major in-vivo adducts produced matched the adduct profile of DNA or dGMP-bis-phenol-o-quinone. Adducts were examined after incubation of DNA with BPA in the presence of the microsomal activation system in an effort to determine how BPA may be converted to DNA binding metabolites. The in-vitro incubation of BPA with DNA in the presence of a microsomal activation system revealed one major adduct and several minor adducts. Known inhibitors of cytochrome-P450 markedly reduced the formation of adducts in DNA by BPA in the presence of a microsomal activation system. Adduct formation in DNA when cumene-hydroperoxide or NADPH was used as a cofactor showed adducts with similar chromatographic mobilities as those from the reaction of dGMP-bisphenol-o-quinone. The authors conclude that BPA is able to covalently bind to DNA both in-vitro and in-vivo, and that covalent alterations in the DNA by in-vivo exposure of BPA may play a role in the induction of liver toxicity. 
BISPHENOL A; DNA ADDUCTS