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5063755 
Journal Article 
Monitoring of glucose and ethanol during wine fermentation by bienzymatic biosensor 
Samphao, A; Butmee, P; Saejueng, P; Pukahuta, C; Svorc, L; Kalcher, K 
2018 
1 
Journal of Electroanalytical Chemistry
ISSN: 1572-6657
EISSN: 1873-2569 
816 
179-188 
WOS:000431156900023 
This research monitored the glucose and ethanol content as quality markers in alcoholic drinks during the wine fermentation process by a bienzymatic biosensor. The biosensor was based on glucose oxidase (GOx) and alcohol dehydrogenase (ADH) chemisorbed on core shell Fe3O4@Au nanoparticles and then subsequently immobilized with the aid of an external magnetic field onto a carbon paste electrode modified with 5% (m:m) manganese dioxide. This biosensor exhibited good linear response for glucose and ethanol from 0.3 to 7.8 mM and 0.1 to 0.7 M. The biosensor was applied to monitoring glucose and ethanol during honey wine fermentation with wine yeast (Sacciwromyces cerevisiae Type II). Samples of 24 must wines were analyzed by the biosensor. Results were in agreement with those obtained by reference methods (commercial glucose meter and gas chromatography), indicating that the biosensor had the potential to be a useful tool to assist winemakers during wine production. 
Glucose oxidase; Alcohol dehydrogenase; Carbon paste electrode; Manganese dioxide; Magnetic core-shell nanoparticles; Bienzymatic biosensor