US EPA

5135451 

Journal Article 

The Suppressive Effects of Benzpyrene on the Manifestations of the Increased Tonus of the Sympathetic Nervous System: the Influencing of the Penetration of Hyaluronidase in the Skin of the Rabbit 

Vrubel, J 

1961 

1 

Neoplasma
ISSN: 0028-2685
EISSN: 1338-4317 

8 

1 

22-26 

English 

The effects of benzpyrene (50328) (BP) alone and in benzene solution on the nervous system was studied in rabbits. Champagne-rabbits had either benzene or benzpyrene in benzene solution applied to depilated hind legs. Group one received 0.5 percent benzene; group two received 0.5 percent benzpyrene in benzene solution; group three received 0.5 percent benzene treatment twice within 4 days, and were narcotized 3 hours after the final treatment; group four received 0.5 percent benzpyrene in benzene solution twice in 4 days. Three hours after final treatment, electrical irritation of the left truncus sympaticus lumbalis muscle was conducted for 20 minutes. Then 0.2 milliliters (ml) of a solution of 1ml hyasa, 3ml saline, and 1ml of 1 percent trypan-blue were injected subcutaneously. Irritation continued for 10 minutes, the animals were sacrificed, and penetration of hyaluronidase was determined. All rabbits showed slightly reddened and edematous skin. The effects of benzpyrene on penetration of hyaluronidase into the skin were about 149, 92, 135, and 96 percent for group one through four, respectively, expressed as a percent of the control side. Benzpyrene in benzene solution suppressed hyaluronidase penetration more effectively than did benzene alone.