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2132467 
Technical Report 
HOST-MEDIATED IN VIVO-IN VITRO ASSAY FOR CHEMICAL CARCINOGENESIS 
Dipaolo, JA; Nelson, RL; Donovan, PJ; Evans, CH 
1973 
Yes 
Archives of Pathology
ISSN: 0363-0153 
ETICBACK/5384 
380 
6 
380-385 
English 
4701393 
HEEP COPYRIGHT: BIOL ABS. A host-mediated in vivo-in vitro combination bioassay for identification of potential carcinogenicity of chemicals for mammalian (hamster embryo) cells is described. The system differs from established quantitative in vitro assays by in vivo transplacental exposure as opposed to direct application of chemicals to cells in culture. Fetuses exposed in utero are subsequently excised. The cells grown in culture and the presence of tranformed cells are determined as in standard in vitro systems by study of colony morphology. Neoplastic transformation is verified by tumor production. None of 5 noncarcinogens produced neoplastic transformation, whereas all 12 chemical carcinogens including several known to be inactive by direct in vitro exposure to cells did so. This in vivo-in vitro system provides a reproducible bioassay for chemical carcinogens, reducing false-negative results that may occur because of the requirement for metabolic activation.