Separation of ten benzo(A)pyrene phenols by recycle high pressure liquid chromatography and identification of four phenols as metabolites | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

5140623

Reference Type

Journal Article

Title

Separation of ten benzo(A)pyrene phenols by recycle high pressure liquid chromatography and identification of four phenols as metabolites

Author(s)

Croy, RG; Selkirk, JK; Harvey, RG; Engel, JF; Gelboin, HV

Year

1976

Is Peer Reviewed?

Yes

Journal

Biochemical Pharmacology
ISSN: 0006-2952
EISSN: 1873-2968

Volume

Issue

Page Numbers

227-230

Language

English

DOI

10.1016/0006-2952(76)90303-8

Web of Science Id

WOS:A1976BG44000029

Abstract

HEEP COPYRIGHT: BIOL ABS. The present report establishes methodology for the separation of benzo(a)pyrene (BP) phenols and demonstrates that 4 of these phenols are BP metabolites. They are the major phenol metabolites and further quantitative studies will determine whether they constitute the total BP phenol formation. The method described can be used to determine the primary catalytic sites of the various forms of the mixed function oxygenases. Highly purified cytochrome P-450's convert BP to phenol peaks with different fluorescent spectra. The new method will help determine the site preference of different mixed function oxygenases for BP hydroxylation and their role in carcinogen activation and detoxification in rat liver microsomes.