Health & Environmental Research Online (HERO)


Print Feedback Export to File
5141548 
Technical Report 
Metabolization of 3.4-Benzpyrene in Mouse Skin Treated with 9.10-Dimethyl-1,2-Benzanthracene 
Norden, G 
1957 
Yes 
Acta Pathologica et Microbiologica Scandinavica
ISSN: 0365-5555 
40 
4 
283-295 
English 
The metabolism of 3,4-benzpyrene (50328) (BP) was investigated in the skin of white mice. Neck hair was shaved from mice and concentrations of 9.10-dimethyl-1,2-benzanthracene (57976) (DMBA) ranging from .005 to 0.5 were applied. At intervals from 4 hours to 12 days, a solution containing 0.5 percent BP was dropped onto the same area. Animals were also treated with DMBA alone or with BP alone. Animals were killed 24 hours after BP application and treated skin was removed and studied by fluorescence microscopy. Fluorescence spectra in DMBA treated animals showed DMBA in epidermis and hair follicles and sebaceous glands on day 1. Skin treated with DMBA showed an increased amount of unchanged BP when treated at 1 to 7 days intervals between chemicals. Epithelial damage occurred after 0.05, 0.15 and 0.25 percent DMBA. Skin in the proliferating phase showed epidermal hyperplasia. The epidermis was damaged in all animals whose skin was in the resting phase; some hair follicles disappeared as did sebaceous glands. Hair follicles grew directly from the epidermis. The author concludes that the difference in reaction of skin regions in resting and proliferation phases may have something to do with differences in quantities reaching the cells.