US EPA

5142825 

Journal Article 

Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation 

Hsu, T; Deng F-Y 

1996 

Yes 

Chemosphere
ISSN: 0045-6535
EISSN: 1879-1298 

33 

10 

1975-1980 

English 

BIOSIS COPYRIGHT: BIOL ABS. The susceptibility of various fish organs to polycylic aromatic hydrocarbons(PAHs)-induced DNA adduct formation was studied in zebrafish using benzo(a)pyrene as the representative carcinogenic PAH. Following exposure of fish to waterborne BaP at 0.2 mg for 3 days and at 1 mg/L for 4 days, 32P-postlabelling analysis indicated that the adduct levels in intestine, liver, brain, and testis DNA were 13.3 | 1.2, 4.3 | 2.5, 3.8 | 0.5, and 0.2 | 0.1 adducts per 108 nucleotides, respectively. When zebrafish were treated with BaP at 0.02 mg/L for 3 days and at 0.1 mg/L for 4 days, a significant increase in the level of bulky adducts was detected only in intestine DNA (0.28 | 0.06 adduct/108 nucleotides), and no adduct spots were observed for DNA isolated from other organs. Our data suggest that the fish intestine is a more sensitive target organ than the liver for biomonitoring the presence of carcinogenic PAHs in the aquatic environment, especially when PAHs are present at low l