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5144679 
Journal Article 
Sequential in vitro effects of airborne particles in lung macrophages and reporter CAT-gene cell lines 
Goegan, P; Vincent, R; Kumarathasan, R; Brook, J 
1998 
1 
Toxicology In Vitro
ISSN: 0887-2333
EISSN: 1879-3177 
12 
1 
25-37 
English 
BIOSIS COPYRIGHT: BIOL ABS. We have studied the responses of a target cell model to culture medium conditioned by lung macrophages exposed to ambient airborne particulate matter. Macrophages were harvested from Fischer 344 rats by broncho-alveolar lavage and incubated in cell culture inserts in the presence of particulate material for 24 hr. The test culture medium outside the insert was then applied to transgenic HepG2 cells containing chloramphenicol acetyl transferase (CAT) genes under transcriptional control from response elements or promoters for xenobiotic-, metal-, cellular stress- and cytokine-responsive genes. The induction of xenobiotic-responsive CAT genes CYP1A1 (cytochrome P4501A1 promoter) and XRE (xenobiotic response element) in the reporter cells by urban dusts correlated with the dose and content in polycyclic aromatic hydrocarbons of the particulate materials added to the inserts. In addition, induction of HMTIIa (metallothionein IIa promoter) by a PM2.5 (aerodynamic size cut-off