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5146485 
Journal Article 
The Effects Of Phenobarbital, 3-Methylcholanthrene And Benzo(a)pyrene On The Hydrolysis Of Xenobiotics In The Rat 
Kaur, S; Ali, B 
1983 
Yes 
Biochemical Pharmacology
ISSN: 0006-2952
EISSN: 1873-2968 
PERGAMON-ELSEVIER SCIENCE LTD 
OXFORD 
32 
22 
3479-3480 
English 
6316983 
WOS:A1983RU04500031 
The effects of phenobarbital (50066), 3-methylcholanthrene (56495) and benzo(a)pyrene (50328) on the activities of esterase/amidase catalyzing hydrolytic metabolism of xenobiotics was investigated in rat tissue. Albino-rats were given 80 milligrams per kilogram of the chemicals intraperitoneally for 6 consecutive days. Animals were sacrificed 18 to 20 hours later and liver, lung, kidney, intestine, and brain tissue were removed. The activities of acetylsalicylic-acid esterase, procaine esterase, p-nitrophenyl-acetate esterase, acetanilid N-deacetylase, and cholinesterase in liver microsomes and tissue homogenates were determined. The rate of 2-acetylaminofluorene hydrolysis was measured. There was marked stimulation of hepatic microsomal carboxylesterases/amidases necessary for hydrolytic metabolism of acetylsalicylic-acid, procaine, p-nitrophenylacetate, acetanilid, and 2-acetylaminofluorene in rats pretreated with phenobarbital. Cholinesterase activity did not change. Enzyme activity in other tissues was not changed. Administration of 3-methylcholanthrene or benzo(a)pyrene did not affect enzyme activity in any tissue. The authors conclude that phenobarbital is a potent inducer of hepatic microsomal esterases and amidases.