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5149468 
Journal Article 
Uptake of Benzo(a)pyrene, Carbaryl, DDT and Parathion in Cultured Human Cells: Re-evaluation 
Murakami, PDN; Fukami J-I 
1979 
Yes 
Bulletin of Environmental Contamination and Toxicology
ISSN: 0007-4861
EISSN: 1432-0800 
Springer-Verlag 
21 
4-5 
478-482 
English 
WOS:A1979HA46800008 
The uptake of benzo(a)pyrene (50328) (BP), carbaryl (63252), dichlorodiphenyltrichloroethane (50293) (DDT), and parathion (56382) by human embryonic lung diploid cultured cells were studied after incubation of near confluent monolayer cell cultures with these chemicals at a concentration of 4 x 10(-6) molar for 4 to 48 hours. The cell monolayers were then immersed in cold 4 percent perchloric-acid for 40 minutes to remove acid soluble materials. After rinsing with 80 and 100 percent ethanol to remove the ethanol soluble fraction, the ethanol insoluble fraction was dissolved in 85 percent formic-acid. Ghost monolayers were prepared by washing monolayers with tris(hydroxymethyl)aminomethane and treating with buffer-A containing 0.5 percent Triton-X-100. The fragile ghost monolayers were digested for analysis using 0.5 percent sodium-hydroxide (NaOH). After incubation for 24 hours, DDT was easily removed from the cells by treatment with ethanol. About 0.2 percent of the insecticide remained in the acid and ethanol insoluble fraction. Thirty times more carbaryl and about six times more parathion as DDT were found in the insoluble fraction. About 40 percent of BP was extracted with ethanol, however, 1 percent was found in the insoluble residue. The amount of association of the chemicals with the ghost monolayers was proportional to the time of incubation. DDT levels were lowest and parathion highest in ghost monolayers, in contrast to their relative uptake into ethanol soluble components. The authors conclude that DDT associated by non specific binding to lipid rich surface membranes, while carbaryl and parathion remained mostly in the insoluble fraction or in the detergent resistant ghosts. This indicated that carbaryl and parathion were more closely associated with cellular components such as proteins.