Health & Environmental Research Online (HERO)


Print Feedback Export to File
5152854 
Journal Article 
Detection of QTLs linked to leaf and smoke properties in Nicotiana tabacum based on a study of 114 recombinant inbred lines 
Julio, E; Denoyes-Rothan, B; Verrier, JL; de Borne, FD 
2006 
1 
Molecular Breeding
ISSN: 1380-3743 
SPRINGER 
DORDRECHT 
18 
1 
69-91 
English 
WOS:000239892600007 
Quantitative trait loci (QTLs) were investigated in a recombinant inbred line (RIL) population descended from a cross between two flue-cured Nicotiana tabacum L. inbred lines with unrelated breeding origins. A total of 59 traits, related to diverse agronomic, leaf quality, chemical composition and smoke properties were assessed. Chemical traits and smoke mutagenicity were estimated by near infrared reflectance spectroscopy (NIRS) analyses of leaf lamina powders. Physical properties of cigarettes made from each RIL, and the total particulate matter (tar and nicotine yields), benzo[a]pyrene and CO contents of the main smoke stream generated by the cigarettes in mechanical smoking tests under a standard ISO regime were also analyzed. The RILs were screened for 184 amplified fragment length polymorphism (AFLP), inter simple sequence repeat (ISSR), sequence specific amplified polymorphism (SSAP), sequence characterized amplified region (SCAR) and biological markers. A partial genetic map including 18 linkage groups was constructed based on 138 of the markers. Substantial segregation distortion (47%) was observed in linkage groups throughout the genome. Seventy-five QTLs associated with 8-41.5% of the variation in the examined traits were identified on 12 linkage groups by simple and composite interval mapping. Nineteen QTLs had opposite effects to those expected from the ranking of parental means. © Springer Science+Business Media B.V. 2006. 
agronomic traits; linkage map; Nicotiana tabacum; QTLs; smoke properties