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5153475 
Journal Article 
UVA light-induced DNA single-strand cleavage by hydroxybenzo[a]pyrenes 
Yu, HT; Dong, SM; Fu, PP; Hwang, HM 
2002 
1 
Polycyclic Aromatic Compounds
ISSN: 1040-6638
EISSN: 1563-5333 
Taylor and Francis Ltd. 
22 
3-4 
861-870 
English 
WOS:000177949300060 
UVA light-induced DNA single-strand cleavage by 1-hydroxy, 3-hydroxy, 7-hydroxy, and 9-hydroxybenzo[a]pyrenes (OH-BaPs) and 6-acetoxybenzo[a]pyrene (6-OAc-BaP) was studied. Under experimental conditions, the concentrations of 1-OH, 3-OH, 7-OH, and 9-OH-BaPs and 6-OAc-BaP needed to cause 25% of the supercoiled form I plasmid DNA to become relaxed form II DNA were found to be 0.6, 2.5, 1.0, 1.3, and 1.1 μM, respectively. These concentrations are all smaller than that of BaP, which was 6 μM. These results indicate that on photoirradiation, OH-BaPs are more cytotoxic and/or genotoxic than their parent compound, BaP. Mechanistic studies reveal that singlet oxygen and superoxide free radicals are involved in causing DNA cleavage. 
benzo[a]pyrene; DNA cleavage; hydroxybenzo[a]pyrenes; light; metabolites; PAH