US EPA

5168379 

Journal Article 

A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose 

Zhao, ZW; Chen, XJ; Tay, BK; Chen, JS; Han, ZJ; Khor, KA 

2007 

Yes 

Biosensors and Bioelectronics
ISSN: 0956-5663
EISSN: 1873-4235 

23 

1 

135-139 

English 

17478087 

10.1016/j.bios.2007.03.014 

WOS:000250325400018 

ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 microA/mA cm2. The low detection limit was estimated to be 20 microM (S/N=3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.