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5187854 
Journal Article 
LABELING OF POLYSACCHARIDES USING [C-11] CYANOGEN-BROMIDE - IN-VIVO AND IN-VITRO EVALUATION OF C-11 HYALURONAN UPTAKE KINETICS 
Westerberg, G; Bergstrom, M; Gustafson, S; Lindqvist, U; Sundin, A; Langstrom, B 
1995 
1 
Nuclear Medicine and Biology
ISSN: 0969-8051
EISSN: 1872-9614 
22 
2 
251-256 
English 
7539321 
WOS:A1995QL60400016 
A method for the 11C-labelling of polysaccharides in high specific radioactivity is described. Dextran and hyaluronan were treated with [11C]cyanogen bromide in aqueous solution at pH 11.5 to give 30-47% radiochemical yields with higher than 98% radiochemical purity in synthesis times of 24-26 min counted from the end of bombardment. Specific radioactivities at the end of synthesis ranged from 0.12 to 3.1 Ci/mumol. The biodistribution kinetics of [11C]hyaluronan injected intravenously was studied in rats by means of positron emission tomography, showing a rapid and displaceable uptake in liver. Uptake and displacement of [11C]hyaluronan was also demonstrated in cultured rat liver endothelial cells.