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5194578 
Journal Article 
Cleavage at met-x bonds by cyanogen bromide 
Simpson, RJ 
2007 
2007 
pdb.prot4704 
English 
21357041 
INTRODUCTIONSelective cleavage of a protein by cyanogen bromide generates a small number of relatively large peptide fragments. This protocol describes protein cleavage with cyanogen bromide, using ~100-fold molar excess of cyanogen bromide over the methionine. The reaction is performed under mild acidic conditions (e.g., 70% formic acid), under nitrogen, and in the dark. All reagents are volatile and can be readily removed by lyophilization.