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5196864 
Journal Article 
The collagenous protein with elastin crosslinks from Descemet's membrane is not related to type VIII collagen 
Davis, PF; Ryan, PA; Kittelberger, R; Greenhill, NS 
1990 
Yes 
Biochemical and Biophysical Research Communications
ISSN: 0006-291X
EISSN: 1090-2104 
171 
1 
260-265 
English 
2393394 
A collagen-like insoluble protein containing the elastin cross-links (desmosine and isodesmosine) has been isolated from Descemet's membrane. Recently type VIII collagen (endothelial collagen) has been shown to be a major constituent of this membrane. Biochemical studies suggest that these two proteins are unrelated. The cyanogen bromide peptide maps show negligible similarity. Antiserum raised against oxalic acid digests of elastin (alpha-elastin) did not react against an oxalic acid digests of type VIII collagen but did show some reaction against the cross-linked preparation. Immunofluorescent localization has demonstrated the presence of type VIII collagen in trachea but a desmosine cross-linked collagen could not be isolated from this tissue.