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5199038 
Journal Article 
In situ cyanogen bromide cleavage of N-terminally blocked proteins in a gas-phase sequencer 
Simpson, RJ; Nice, EC 
1984 
Yes 
Biochemistry International
ISSN: 0158-5231 
8 
6 
787-791 
English 
6089832 
WOS:A1984SZ55900006 
Herein we describe a procedure for the in situ cyanogen bromide cleavage of N-terminally blocked proteins which have been immobilised onto the glass fiber sample disk of the gas-phase sequencer. In this manner, new amino terminii suitable for automated Edman degradation can be generated. Cytochrome C was cleaved using this method on the carboxyl side of methionine residues 65 and 80. This allowed sequence analysis to begin simultaneously at residues 66 and 81 (Table 1). This procedure offers an alternative sequencing tactic for methionine-containing proteins which are N-terminally blocked.