Amino-acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan (Oxyuranus s. scutellatus) | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

5199746

Reference Type

Journal Article

Title

Amino-acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan (Oxyuranus s. scutellatus)

Author(s)

Lind, P; Eaker, D

Year

1982

Is Peer Reviewed?

Yes

Journal

European Journal of Biochemistry
ISSN: 0014-2956
EISSN: 1432-1033

Volume

124

Issue

Page Numbers

441-447

Language

English

PMID

7049694

Abstract

The amino acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan has been determined. The very basic protein, by itself a moderately neurotoxic phospholipase, consists of a single polypeptide chain of 119 amino acids. The main fragmentation of the reduced and S-carboxymethylated derivative was accomplished by cleavage with Staphylococcus aureus V8 protease and trypsin. Chymotryptic peptides and cyanogen bromide fragments were used to align and complete the sequence, which was determined by automated Edman degradation. The taipoxin alpha-subunit is closely homologous to the other taipoxin subunits and to other elapid snake venom phospholipases A2.