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520049 
Journal Article 
Stability of haemoglobin A(1c) (HbA(1c)) measurements from frozen whole blood samples stored for over a decade 
Selvin, E; Coresh, J; Jordahl, J; Boland, L; Steffes, MW 
2005 
Yes 
Diabetic Medicine
ISSN: 0742-3071
EISSN: 1464-5491 
22 
12 
1726-1730 
English 
Objective Haemoglobin A(1c) (HbA(1c)), a measure of long-term glycaemic control, is at the centre of the clinical management of diabetes mellitus. However, the reproducibility of HbA(1c) measurements from whole blood samples which have been in long-term storage is unknown. We undertook this study to assess the reproducibility of HbA(1c) measurements from whole blood samples that had been in storage at -70 degrees C for over a decade. Research design and methods Three hundred and thirty-six samples of frozen whole blood from the Atherosclerosis Risk in Communities (ARIC) Study, stored at -70 degrees C for 11-14 years assayed for HbA(1c) using a dedicated ion-exchange HPLC assay (Tosoh A(1c) 2.2 Plus HPLC) were compared with measurements on these same samples conducted prior to storage (in 1990-92) using a Diamat (Bio-Rad) HPLC instrument. Results HbA(1c) measurements from long-term stored samples were strongly correlated with values obtained prior to long-term storage (r = 0.97). The difference between HbA(1c) from long- and short-term stored samples had a mean of 0.35% HbA(1c) (SD = 0.35) and a CV of 5.8%, which was approximately three times that of duplicate assays (CV 1.3 to 2.5%). Conclusions These data demonstrate that highly correlated but more variable and slightly higher HbA(1c) results were obtained from frozen whole blood samples that have been in storage for more than a decade. This highly reproducible assay performance would lead to comparable ranking of individuals and unbiased estimates of relative risks and odds ratios in epidemiological studies (case-control and cohort designs), but results should be realigned when the absolute value is of interest. These results have important implications for epidemiological studies and clinical trials which have stored whole blood specimens. 
HbA(1c); diabetes; reliability; long-term storage; stored whole blood; glycohemoglobin standardization program; atherosclerosis risk; glycated; hemoglobin; diabetes-mellitus; complications; individuals; communities; regression; level; men