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5205768 
Journal Article 
Acute and Repeated Treatment with 5-PAHSA or 9-PAHSA Isomers Does Not Improve Glucose Control in Mice 
Pflimlin, E; Bielohuby, M; Korn, M; Breitschopf, K; Löhn, M; Wohlfart, P; Konkar, A; Podeschwa, M; Bärenz, F; Pfenninger, A; Schwahn, U; Opatz, T; Reimann, M; Petry, S; Tennagels, N 
2018 
Yes 
Cell Metabolism
ISSN: 1550-4131 
28 
2 
217-227.e13 
English 
29937376 
WOS:000440983900007 
Fatty acid esters of hydroxylated fatty acids (FAHFAs) were discovered as a novel class of endogenous mammalian lipids whose profound effects on metabolism have been shown. In the current study, in vitro and in vivo the metabolic effects of two of these FAHFAs, namely palmitic acid-5- (or -9) -hydroxy-stearic acid (5- or 9-PAHSA, respectively) were profiled. In DIO mice fed with differentially composed low- or high-fat diets, acute and subchronic treatment with 5-PAHSA and 9-PAHSA alone, or in combination, did not significantly improve the deranged metabolic status. Neither racemic 5- or 9-PAHSA, nor the enantiomers were able to: (1) increase basal or insulin-stimulated glucose uptake in vitro, (2) stimulate GLP-1 release from GLUTag cells, or (3) induce GSIS in rat, mouse, or human islets or in a human pancreatic β cell line. Therefore, our data do not support the further development of PAHSAs or their derivatives for the control of insulin resistance and hyperglycemia.