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5208609 
Journal Article 
Influence of extractive solvents on lipid and fatty acids content of edible freshwater algal and seaweed products, the green Microalga Chlorella kessleri and the Cyanobacterium Spirulina platensis 
Ambrozova, JV; Misurcova, L; Vicha, R; Machu, L; Samek, D; Baron, M; Mlcek, J; Sochor, J; Jurikova, T 
2014 
Molecules
ISSN: 1420-3049 
19 
2344-2360 
English 
Total lipid contents of green (Chlorella pyrenoidosa, C), red (Porphyra tenera, N; Palmaria palmata, D), and brown (Laminaria japonica, K; Eisenia bicyclis, A; Undaria pinnatifida, W, WI; Hizikia fusiformis, H) commercial edible algal and cyanobacterial (Spirulina platensis, S) products, and autotrophically cultivated samples of the green microalga Chlorella kessleri (CK) and the cyanobacterium Spirulina platensis (SP) were determined using a solvent mixture of methanol/chloroform/water (1:2:1, v/v/v, solvent I) and n-hexane (solvent II). Total lipid contents ranged from 0.64% (II) to 18.02% (I) by dry weight and the highest total lipid content was observed in the autotrophically cultivated cyanobacterium Spirulina platensis. Solvent mixture I was found to be more effective than solvent II. Fatty acids were determined by gas chromatography of their methyl esters (% of total FAMEs). Generally, the predominant fatty acids (all results for extractions with solvent mixture I) were saturated palmitic acid (C16:0; 24.64%-65.49%), monounsaturated oleic acid (C18:1(n-9); 2.79%-26.45%), polyunsaturated linoleic acid (C18:2(n-6); 0.71%-36.38%), α-linolenic acid (C18:3(n-3); 0.00%-21.29%), γ-linolenic acid (C18:3(n-6); 1.94%-17.36%), and arachidonic acid (C20:4(n-6); 0.00%-15.37%). The highest content of ω-3 fatty acids (21.29%) was determined in Chlorella pyrenoidosa using solvent I, while conversely, the highest content of ω-6 fatty acids (41.42%) was observed in Chlorella kessleri using the same solvent. 
; Freshwater environments; Lipids; Methanol; Solvents; Arachidonic acid; gamma -linolenic acid; Seaweed products; Substance P; Chloroform; Gas chromatography; Palmitic acid; Fatty acids; Oleic acid; n-Hexane; Algae; Linoleic acid; Hizikia; Cyanobacteria; Palmaria palmata; Laminaria japonica; Chlorella kessleri; Chlorella pyrenoidosa; Porphyra tenera; Spirulina platensis; Undaria pinnatifida; Eisenia bicyclis/