Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
527859
Reference Type
Journal Article
Title
Genetic identification of an embryonic parafacial oscillator coupling to the preBotzinger complex
Author(s)
Thoby-Brisson, M; Karlen, M; Wu, N; Charnay, P; Champagnat, J; Fortin, G
Year
2009
Is Peer Reviewed?
1
Journal
Nature Neuroscience
ISSN:
1097-6256
EISSN:
1546-1726
Volume
12
Issue
8
Page Numbers
1028-U1100
Language
English
DOI
10.1038/nn.2354
Abstract
The hindbrain transcription factors Phox2b and Egr2 (also known as Krox20) are linked to the development of the autonomic nervous system and rhombomere-related regulation of breathing, respectively. Mutations in these proteins can lead to abnormal breathing behavior as a result of an alteration in an unidentified neuronal system. We characterized a bilateral embryonic parafacial (e-pF) population of rhythmically bursting neurons at embryonic day (E) 14.5 in mice. These cells expressed Phox2b, were derived from Egr2-expressing precursors and their development was dependent on the integrity of the Egr2 gene. Silencing or eliminating the e-pF oscillator, but not the putative inspiratory oscillator (preBotzinger complex, preBotC), led to an abnormally slow rhythm, demonstrating that the e-pF controls the respiratory rhythm. The e-pF oscillator, the only one active at E14.5, entrained and then coupled with the preBotC, which emerged independently at E15.5. These data establish the dual organization of the respiratory rhythm generator at the time of its inception, when it begins to drive fetal breathing.
Keywords
congenital central hypoventilation; respiratory rhythm generation; pre-botzinger complex; fatal central apnea; neonatal-rat; developing; hindbrain; nervous-system; mouse embryo; in-vitro; neurons
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity