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530216 
Journal Article 
Effect of various antioxidants on the quality of frozen-thawed bull semen 
Uysal, O; Bucak, MN; Yavas, I; Varisli, O 
2007 
6 
12 
1362-1366 
English 
Free radicals are known to be involved in lipid peroxidation as well as DNA and sperm membrane damages that may lead to decreased sperm motility or cell death. The aim of this study was to determine the effects of the addition of various of the supplements consist of glutathione (GSH), oxidized Glutathione (GSSG), cysteine, taurine, hypotaurine, Bovine Serum Albumin (BSA), trehalose and hyaluronan to freezing media on the post-thawing sperm characteristics, including motility, morphology, viability by propidium iodide (PI stain) with the aid of fluoresans microscope and membrane integrity by HOST. A total number of 20 ejaculates were collected using the artificial vagina from 2 bulls and the ejaculates were diluted with a Tris-based extender containing additives and no additives as control. GSH (5 mM), GSSG (5 mM), cysteine (5 mM), taurine (50 mM), hypotaurine (25 mM) and BSA (5 mg mL(-1)), trehalose (50 mM) and hyaluronan (1000 mu g mL(-1)) showed more positive effect than other supplements and controls in protecting sperm characteristics after the freezing-thawing process (p<0.001). Many aspects of sperm protection e.g., sperm motility, viability and membrane stabilisation of the sperm cells during relative cryopreservation, are the key factors in determining the preservation of sperm function. The results of this study provide a new approach to the cryopreservation of sperm from bulls and related breeds. Further studies are necessary to obtain results to confirm present findings. 
antioxidants; bull semen; freezing; frozen-thawed; BSA; swelling-eosin test; sperm motility; lipid-peroxidation; ram; spermatozoa; blood-serum; trehalose; taurine; glutathione; hyaluronan; viability